Purdue Cytometry Mailing List
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Starting: Wed Jan 02 2002 - 11:15:00 EST
Ending: Tue Dec 31 2002 - 11:38:04 EST
- "Good" anti-goat antibody
- "I"SAC - restore Interenational into the SAC organization.
- (no subject)
- * Current protocols * : given away
- * Current Protocols in Immunology 1994 for free *
- 1024 vs 256 channels
- 15th ANNUAL CLINICAL COURSE IN FLOW CYTOMETRY
- 2 flow cytometry job openings at Biogen
- 2 questions
- 2-me in culture media:summary
- 256 vs. 1024 channels
- 256 vs. 1024 channels Hmmmmmm
- 25th Annual Course in Flow Cytometry
- 25th ANNUAL COURSE IN FLOW CYTOMETRY: BOWDOIN COLLEGE, BRUNSWICK MAINE - JUNE 2002
- 2nd CGES Meeting in Myrtle Beach
- 2nd Conference on Analysis of Microbial Cells at the Single Cell Level
- 2nd EWGCCA EuroConference, Urbino, Sep.19-22,2002
- 3D
- 4-color leuk analysis
- 4-color leukemia panels
- 4th Edition of Practical Flow Cytometry - Last Call
- 523nm laser pointer
- 6-His-tag Mab
- 7-AAD/PE problem
- 7AAD link
- 8. Leipziger Workshop, 1st Announcement
- 8. Leipziger Workshop, 2nd Announcement
- [7AAD viability for CD34]
- [aerosol containment]
- [anti-sheep CD117?]
- [Antibody against human btk?]
- [Blastogenesis Protocol?]
- [CBA Kits]
- [CD45/CD14 monkey peripheral blood]
- [CD55, CD59 on erythrocytes?]
- [CD8 after EtOH fixation]
- [Cell Cycle arrest and pre-synchronization]
- [cell synchrony technique]
- [Clinical Cytometry Society Annual Meeting]
- [Clinical flow sample triage]
- [Clinical lab with third-party maintenance?]
- [Conjugated ABs to human TLR2 and TLR4]
- [Free Seminar on Luminex]
- [FRET Proof?]
- [Fwd: Paul Jolicoeur]
- [Fwd: sorting for mouse DC and Mac]
- [GFP Retention]
- [HIV Back-titration?]
- [Integrin alpha 7 antibody search]
- [intracellular cytokines]
- [Job Listing - Beckman Coulter]
- [localization within lymphocytes]
- [Macintosh Setup]
- [monomeric avidin]
- [Partec CyFlow]
- [PATOL] Un microscopio virtual [LBC-UC6G2C4]
- [Preparation and holding time limits?]
- [Preservative vs Stability]
- [Solid Tumor dissociation]
- [Solvent question]
- [Sorting on diffuse vs punctate?]
- [TdT and Cytoplasmic CD3]
- [Thanksgiving Greeting (U.S.)]
- [TLR2 and 4 on murine Langerhans Cells?]
- [workshop at ISAC]
- A Career Opportunity at Molecular Probes
- A good scientist...
- a more question on cell cycle analysis with PI
- A query?
- A question of PI or DAPI
- a question on cell cycle analysis with PI
- A suggestion
- Ab anti-BRCA1 and BRCA2
- Ab for rat erythrocytes?
- AB's for human perforin, granzymes
- ab's for tamarind cd4/cd8
- ABC transporter
- About calcium determination
- About Symposium & Workshop on Faces and Phases of Dendritic cells
- Abs to human IgG4- which?
- Academic position in CTL-mediated apoptosis
- Accu-Drop/FacsVantage Problem
- ACDU motor
- Acridine Orange and Sperm Chromatin Structure
- acrosome integrity by flow
- activation markers
- advertising
- advertizing - no need to comment on this please.
- Advice on a patient
- Advice on the best assays
- AFCG annual scientific meeting
- afcg meeting
- AFCG meeting, Hobart
- After-the-fact software compensation
- Alexa 532 excitation off an argon 488 laser
- Alexa reds and HeNe (was Antibody Conjugation Vendor)
- alexa-phalloidin
- Algorithms for cell cycle analysis
- An unusual clinical case: seeking clinical opinions
- Analysis of fluorescent timer protein [DC-169FF54]
- Andy Rawstron
- Annexin and fibroblasts
- Annexin V staining of mouse blood lymphocytes
- AnnexinV on RBC after O.N. at 4 C
- Announcing Cytometry CD VOL 6
- Announcing User Forum at Verity Software House, Inc.
- Annual Clinical Cytometry Course Announcement
- ANSWER TO EXPORT RAW DATA
- Anti FITC antibody
- Anti nuclear factor antibody
- anti RAT CD34 and anti RAT sca1
- anti-bax
- anti-CCR7
- Anti-CD3 and tetramer staining
- Anti-CD69 mAbs
- Anti-D
- Anti-D Immunoglobulin prophylaxis and anti-D reagent
- anti-dog CD62L
- anti-dog CD62L revisited
- Anti-human IL4, and IL10 mAbs
- anti-human lymphocyte Ab
- anti-IFN-g
- anti-IgG4 responses
- anti-malaria antibodies
- anti-malarial antibodies
- Anti-mouse CCR7
- anti-mouse CD ab fail to react with wood mice blood cells
- Anti-mouse CD-36 antibody
- anti-salmonella antibodies
- anti-STAT staining
- anti-TCR alpha beta on fixed human cells
- Antibodies
- antibodies into viable cells
- antibodies to human Ig isotypes
- antibodies to mouse J chain
- Antibodies to rabbit atherosclerotic cells types
- Antibody Conjugation Vendor
- Antibody Conjugation Vendor and Alexa633
- antibody for mouse stromal cells
- Antibody for the ABC transporter
- antibody quest
- antibosies against mouse topoisomerase
- anticyclin D, CD15
- antigen specific T cells
- Antwort: a more question on cell cycle analysis with PI
- Antwort: Apoptosis detection
- Antwort: consistency in counting cells by flow
- Antwort: Kits for Apoptosis detection
- Antwort: simultaneous screening of 2 cell lines
- Any Recommendations?
- Anyone used Gel Microdrop technology
- AO/PI viability
- APC & Cy-Chrome
- APC conjugated secondary antibody
- APC-Cy7
- Apoptosis
- Apoptosis and cell cycle analysis
- Apoptosis detection
- Apoptosis in spleen
- Apoptosis kit for granulocytes available
- Apoptosis light scatter detection
- apoptosis may have many faces
- apoptosis measurement for HeLa cells?
- Apoptosis with PI
- Apoptotic assay for fibroblasts
- aquatic muscles
- aquisition by CQ3.3 in Mac OSX
- ar -> kr conversion
- Archeological Data Retrieval
- Arumugham Raghunathan [ragoor@molecularstaging.com] CD4 on granulocytes
- ASH Flow Brewpub Night
- ASH Meeting
- ask for help
- Assistance wanted to debug open source (statistical) FACS software
- Associate Lab Manager position
- Association of Medical Laboratory Immunologists 15th Annual Meeting
- ATTN: Cytometry Facility Core Managers - Scheduling
- Auto fluorescence summary
- Auto-fluorescence
- Auto-fluorescence of NG-108 and PC-12
- Autofluorescence
- autofluorescence in OCT mounted frozen sections
- autopsy material
- availablity of anti-murine CD179a
- AW: cytometry list CD8
- AW: Part time flow position available - Australia
- AW: yeast contaminations
- AZIDE - & QUESTIONS REGARDING FACS IN KUWAIT
- Azide?
- B cell antibodies
- b-mercaptoethanol in media
- B.D versus EXCEL
- bacteria sorting
- Bacteria sorting ?
- Bacteria sorting-follow up
- Bacteria sorting-thanks
- Bacteria sorting?
- Bacteria sorting? Size of droplet etc
- bacteria viability
- Bacterial Analysis
- Bacterial Analysis/Mitochondria
- bacterial calibration beads
- bacterial sorting
- bal and smokers
- Bascteria sorting ?
- Basic S-Phase Question
- Basophills
- Basophills summary
- BBC "documentary"
- BBC Documentary
- Bcl-2 measurement by flow cytometry
- bcl2 and p53 and Mg
- BD Biosciencers Job Opportunity
- BD Biosciences Job Opportunity
- BD Cytokine bead assay
- BD FACSVantage Sorter for sale
- BD LSR
- BD LSR - correction
- BD LSR optics
- Bead assay for detection of viral infection
- beads for quantitation
- Beckman Coulter Job Opportunity
- Best concentration of formaldehyde for phenotyping
- Best concentration of formaldehyde responses
- best markers for human monocytes
- Best protocol for yeast cell cycle?
- billing
- Billing advice
- Biotinylation reagents
- Blocking
- Blood platelet activation
- Bone marrow filters
- Bone Marrow Tech position
- Boston Probes
- bovine DC cell/Macrophage
- BrdU and GFP
- Brdu and Irdu
- brdu in mouse blood
- Breast cancer marker in bone marrow
- breast cancer proliferative rate
- BREfeldin A - Does it fluoresce?
- brefeldin A vs monensin
- Brightness of dyes
- Broken connector on FACScan
- Broken connector on FACScan [TS-EQ84C14]
- c-FLIP and CCR7
- Ca flux in mice protocol
- Ca-indo and gfp?
- Calcium determination by flow cytometry
- Calculating genome size from flow cytometry
- Calibur Maintanence
- Calibur maintenance
- Calibur sheath filter
- calibur vs cytomics FC500 vs CyAn
- Can't get BrdU labeling
- Cancer from PI?
- canine cd-4
- canine cd8
- Canine NK cells
- CAP survey responses
- carbohydrate detection
- Cardiomyocytes and flow
- Caspase 8 via Flow?
- Caspase Measurement
- caspase-8 by flow cytometry
- CD burners
- CD burners: summary
- CD Marker Reference Ranges
- CD-marker-analysis
- CD10 and CD34
- CD105 on fibroblasts
- CD123 antibody for Hairy cell leukaemia
- CD146
- CD178
- CD19 and IgD recommendations
- CD2 expression on memory and naive T cells
- CD23
- CD23 staining of B cells
- CD25 antibody
- CD27 and CD62L
- CD27 and CD62L ALSO - other CD's missing after thawing
- CD3 counts
- CD3 Molecules per Leucocyte?
- CD34
- CD34 cv
- CD36 on human monocytes
- CD38 (OKT10 clone)
- CD38 in CLL
- CD4 absolute count
- CD4 in granulocytes
- CD4 on stimulated human T cells
- CD45 Antibody for baboon
- CD5+CD19+ Normal B cells
- CD52 availability?
- CD52 in patients with CLL
- CD56 in rhesus
- CD6 - Cytomics Issue
- CD6- Final Notice
- CD69 (follow-up, more information)
- CD69 staining
- CD7+CD56+surface-kappa(!!!) positive case
- CD79
- CD8 cell lines
- CD8 enigma... Will it take a village? (Part 1)
- CD8 Epitopes
- CDC ORISE position
- CDs are burning
- Cell cycle analysis
- Cell cycle analysis of neurons
- Cell cycle analysis programme
- cell cycle analysis with GFP and Hoechst not working
- Cell Cycle Programs
- CELL DEATH
- Cell detachment medium (was: EVC 30
- Cell detachment medium (was: EVC 304 abort rate)
- cell line for oxidative burst studies
- Cell line info
- Cell Lines
- cell lines in flow cyt
- cell preparation
- Cell sizes
- Cell Sizes, FSC, and SSC
- cell sorter operator position available
- cell study
- Cell surface properties
- Cell-Dyn 4000
- Cellquest and OS10.1.3
- CellQuest and OSX
- Cellquest for OSX?
- CellQuest Pro
- CellTracker Green Loading
- CFDA-SE staining
- CFSE
- CFSE 4-color
- CFSE analysis by flow
- CFSE and K562 cells
- CFSE labeling for in-vivo vs. in-vitro experiments?
- CFSE Population Identification in a Mixed Cell Population (blood) using Flow Cytometry
- CFSE PROTOCOL
- CFSE PROTOCOL - ANSWER
- CFSE staining problem with PBMC
- CGES 2nd Meeting
- CGES Meeting Agenda and Details
- cheap trick
- Chemokine research center
- Chesapeake Cytometry meeting-06/24/02
- Chesapeake meeting Date Correction
- chicken blood
- Chimpanzee Reference ranges
- Chlamydia antibodies
- chondrocyte viability
- Chromagen and Alexafluor
- Chromosome Sorting
- Chromosome sorting in Australia
- cirque at ISAC
- Cirque Du Soleil comes to ISAC XXI in San Diego
- CLARIFICATION: carbohydrate detection
- Classes?
- Clenz
- clinical billing issues
- Clinical controls
- Clinical Cytometry Society meeting Workshop
- Clinical Cytometry Society Society Meeting
- Clinical Cytometry Society Workshop (apology)
- Clinical Test Nomenclature
- Clinical Test Nomenlature
- Cloning in the US
- CMV antigen
- Coherent 200mW Laser
- Coincidence on Facscan
- Coincidence on facscan...adendum
- Collecting large numbers of events on a Coulter
- Comment on the CD22 expression
- Commercial shenanigans
- Commercial Tutorial - Cy5.5 Tandem Dyes
- Common carrier cells for rare events
- comparing protein expression in varied cell lines
- Compensation
- Compensation ... in microscopy
- Compensation question
- Composition of Coulter Clenz
- computer network
- Computer Networks
- conclusions to the ELISPOT interest inventory
- Confocal Microscopy Workshop at ISAC
- Confocal System for Sale
- congratulation for iranian eid-e norooz
- Congress on "Analysis of Microbial Cells at the Single Cell Level"
- Conjugating IgM
- connexin hybridoma
- consistency in counting cells by flow
- Consumer camera to eyepiece adaptation
- contracting services
- controls
- Converting Flow Data
- Converting Flow Data, WinMDI and Ca influx
- Core confocal facility in the Boston area?
- Core Manager's Workshop
- Core operator position - AIDS Research Center, Mass. General Hosp ital, Boston
- Core operator position - AIDS Research Center, Mass. General Hospital, Boston
- COS cells
- Coulter Epics Profile II
- Counting murine monocytes in blood
- Course for Clinical Flow Cytometry
- Course in UK
- courses
- Courses in UK
- cox-2 antibody
- CPT tube
- Crystal violet on macrophages.
- CSF Immunophenotyping
- Current Protocols
- Current status of RFlowCyt
- Custom GMP Antibodies
- CVID patient, protocol for CD40L / LPS activation of Bcells ?
- cy3 fixation
- CYAN
- CYAN ????
- CyChrome Hoechst question
- Cyclin D1 Method
- Cytek AMS 96 well sampler
- Cytokine bead kits
- Cytokine increases autofluorescence!?
- Cytokine measurement in transfected cell line
- cytokine reference sheet
- cytokines from mouse blood
- Cytometer
- cytometrists in El Salvador
- Cytometry CDROM volume 6
- Cytometry Course in Portugal
- Cytometry online?
- Cytometry School, Krakow - First Announcement
- Cytomics
- Cytomics Info - CYTOMETRY LIST MEMBERS
- Cytomics' Meeting: Advanced Techniques in Analytical Cytology VII
- Cytoplasmic mu
- Cytospin 3
- Daily Compensation in Clinical Flow Lab
- DAPI staining protocol
- darned autofluorescence
- darned autofluorescence]
- Data Management
- Data Management addition
- data question
- data question - omitted info
- Data transformation
- DC-SIGN Ab
- Dead cell detection and intracellular cytokine staining
- Dead cells and sorting
- dead tech rep society
- deadline approaching for the 15th Annual Clinical Course in Flow Cytometry!
- DEADLINE OCT 24 FloCyte Regional Training Program in San Diego
- Dee, summary macrophage, monocye harvesting
- degranulation-another question!
- delta version versus Leica confocal
- Dendritic cell apoptosis
- Dendritic cells
- Descriptive statistics
- Detaching cells off TC plate after fixation
- detecting autoantiboy on cell surface (human)
- Detecting not activating CD3
- detection of apoptosis
- Detection of different subphases of mitosis by flow cytometry
- Detection of GFP in tissue sections
- Detection of Nanoparticles
- detection of PMN phagocytosis by flowcytometry
- differences in blood collection
- different count in immunocytochemistry and FACS
- differential scanning calorimeter information request
- DiOC / PI assay
- Diphtheria Toxin
- discordant results from flow and microscopy
- discussion re current antibody panels
- Distance Learning
- Diurnal variations for CD4 count
- DiVa software version?
- DNA analysis
- dna analysis controls
- DNA comparison
- DNA cytometry and doubling time
- DNA damage and flow cytometry
- DNA flow Analysis
- DNA profiles of splenocytes
- DNA stain for malaria
- dot plot pattern
- Dow Corning Silastic Tubing
- Down regulation of CD4 in BAL
- DRAQ5
- DRAQ5 Revisited
- DRAQ5-Thanks
- DRAQ5: may be an alternative for Hoechst 33342 if I have only 488nm argon laser?
- Drosophila
- dsRNA and ethanol fixation
- DX5 and morphology
- dye laser details
- Dye to use
- EDTA to help unstick sticky cells
- EDTA tubes
- Effect of PFA
- EGFP-EYFP fusion
- Electronic signature software
- ELISPOT interest inventory and name change CLB
- Elite 2-D HST files
- EM after permeabilization
- EMAIL ABUSE - how to stop
- EMBL-Vacancy
- Employment Opportunity
- Endothelial cell detachment
- Endothelial cell detachment SUMMARY
- Endothelial cell surface marker
- endothelial cells
- Endotoxin removal
- energy transfer
- Enzymatic Amplification Staining
- Eosinophil leukaemia?
- eosinophil nonspecific binding
- EPIC SALE
- Equipment for sale
- Erythrocytic Stem Cells
- erythroleukaemia
- europe
- EVC 304 abort rate
- Exciting Development Position Available
- export files from CellQuest
- EXPORT RAW DATA-clarification of request
- EXPORTING RAW FCS DATA
- EXPORTING RAW FCS DATA with WinList 5
- FACS antibodies
- FACS DiVa impressions wanted
- FACS facility operator required in Melbourne, Australia.
- FACS on peritoneal lavage
- FACS-analysis of muscle cells from tissue sections
- FacsCaliber for sale
- FacsCalibur Diode Laser Problems - update
- FACSCalibur fluorescence sensitivity
- FACSCalibur Red Diode Laser Reliability
- FACScan for Sale
- FACScan price???
- facscan wanted
- FacsCount
- FacsCount - seeking training
- Facsimile: Flow teaching software & source available
- Facstar plus part
- FACT accreditation
- Fanning - was GFP sorting question
- Fast Blue
- Fc block/non-specific block
- Fc receptor blockade in human cells
- Fc-Blocking
- FcReceptors on HMNC
- FCS assistant
- FCS Data File Archives
- FCS Express Version 2
- FDA Regulation and cytometry
- Feline NK cells
- FIBROBLAST MARKER
- Field Technical Specialist Position Announcment
- File Gaurd 4.1
- FISH and Flow
- FISH and flow cytometry
- FITC and PI compensation
- FITC vs Alexa 488
- FITC vs Fluorescein-EX conjugation
- FITC-labelling of bacteria
- five cents on ATP detection
- fix first stain later for surface antigens
- fixation buffer
- fixing permeabilized cells
- FL1-FL3 compensation.
- flocyte basic training course at Danbury ct
- Flocyte Flow Cytometry Course in Tampa, Florida!
- FloCyte Regional Basic Training Program
- Flow Club
- Flow Core Billing
- flow course announcement
- flow course or practice
- Flow cytometer comparison
- Flow Cytometrist Position at NIH
- Flow cytometrist position in NYC
- flow cytometry and LPA
- Flow Cytometry classes
- Flow Cytometry Course
- Flow Cytometry Employment Opportunity
- Flow Cytometry ICD9 codes
- Flow Cytometry Manager position
- Flow Cytometry meeting in Minneapolis, MN
- Flow cytometry on skin cells
- Flow Cytometry Operator position
- Flow Cytometry position
- Flow Cytometry Position, Dallas
- Flow cytometry with E.coli tagged with GFP
- Flow Facility Rates in Canada
- Flow GLP Validation
- Flow Jo for PC
- Flow on Nematode eggs
- Flow position available- Scotland
- Flow position in London, Ontario, Canada
- Flow rate question
- flow sensitivity
- Flow sorting of chromosomes to make genomic libraries
- flow+nmr= ?
- Flowcell in a FACStar Plus?
- Flowcytometer troubleshooting flow chart
- Flowcytometry or Haematology position wanted in Maryland
- FlowJo 4 opinions wanted
- FlowJo 4.0.2 problem
- FlowJo Analysis Seminar
- FlowJo Announcements
- Fluorescence enhancement
- Fluorescence in Log scale. Why ?
- fluorescence polarization
- Fluorescence Quantitation
- Fluorescence sensitivity FACScan vs. FACS Vantage SE
- Fluorescence sensitivity FACScan vs. FACS Vantage SE [EV-UASEJR3]
- Fluorochromes choice
- fluorochromes in food
- FMC7 purified
- follow up to a previous message about reagents
- Follow Up: Bacterial Analysis
- For NIH flow cytometry users
- For Sale
- For Vantage(Diva) users #1 - beads?
- For Vantage(Diva) users #2 - biohazard containment
- Forensic & flow cytometry
- Found: Joe Chrest!
- Free Bernoulli disks!
- Free mac program for FCM analysis
- Free Software Available
- Freezer sample storage database
- FRET by flow
- FRET Combinations
- FRET frets...
- frog antibodies
- Frog cell sorting
- Frozen Antibodies
- fully functional Elite ESP for sale
- FWD-Job Posting in Cytometry
- Fwd: Dimers, Tetramers, Tracking Dyes, Workshop ISAC XXI
- Fwd: Macintosh - PC networking
- Fwd: Perforin mAb
- g/d t cell detection in mice
- Gating
- Gating apoptotic sub-G1 cells
- Gating for apoptosis studies
- getting antibodies into viable cells
- Getting antibody into viable cells
- GFP along with IL-2 intracellular staining
- GFP compensation
- GFP expression of sorted cells
- GFP in U937?
- GFP rats
- gfp sorting
- GFP Sorting question
- gfp spectral shifts?
- GFP transfection
- glass wall columns
- glia
- glia and flow?
- GLP protocols
- Glut-4
- Golf Anyone?
- Good rabbit polyclonal Ab to cell surface marker
- Gport serial adapter
- Granulocytes fr monocytes by Flow
- Granulocytes from monocytes by Flow
- Granzyme B ELISPOT for cytometry mailing list
- granzymes Flow and ELISPOT and "free" advice
- granzymes Flow and ELISPOT and free samples
- graphics for presentations
- Guava flow cytometer at NIH
- Guinea Pig IF gamma detection
- Gut epithelial cell prep
- HA binding protein question
- halftime clinical cytometry position / Boston
- Happy Holidays from the Boston Users Group for Cytometry
- Hardwareproblem
- harvesting cultured macrophages
- HC2 antibody for Hairy cell leukaemia
- HcRed (w/568 nm excitation?)
- help - fixative issue?
- Help needed in MDR Study
- help please
- Help to interplate some intriguing flow cytometry data
- Help with Biotinylation
- help with dotplots
- Help!
- hemolymph
- Hep B infected Sorting in UK?
- heparinase
- Hepatocyte isolation protocol
- Here I am at the alter of FLOW
- High FITC staining induced FL3 downward shift.
- Historically significant FACS II available
- HIT Assay by flow cytometry
- HIT Heparin Immune thrombocytopenia
- HLA expression on stromal cells.
- HLA FCXM Query - to dilute or not dilute?
- HLA-A2 antibodies
- HLA-ABC blocking antibodies
- HLA-B27 using real time PCR method
- HLA-C
- HLA-E antibody
- HLA-E specific mab
- HLA-FCXM query
- HLA/Tissue typing job
- HLDA8, Myeloid Section
- HMGCoA-synthetase antibodies
- HO33342 into 670/20 filter
- HO33342 into 670/20 filter???
- Hoechst / Pyronin Y "Drift
- Hoechst 33342
- Hoechst 33342 and cell cycle sorting
- Hoechst and APC
- Hoechst staining for FACS Vantage
- Hoechst, GFP, and cell cycle post error
- Hoechst,GFP,cell cycle recent discussion
- Hotel at ISAC
- how many isotype controls?
- How to distinguish the lymphoma cell and the normal cell in FCM?
- how to do FACS analyssis of hematopoistic cell lines?
- How to enhance PE signal?
- How to enhance PE signal? or fluorochromes in general
- How would you interpret this?
- HP computer to a good home
- HP340 and ACDU (facs vantage)
- hp340 to mac
- HP340 to mac : the answer
- human anti-CCR7
- Human CD8 antigen specificities
- Human IgE
- human IL-15 mAbs
- Human IL-2 for ELISPOT
- human NKT cells
- I-90 laser problem
- ICOS
- identifying alveolar macrophages
- identifying human immunoregulatory T cells
- IFCC Course and Symposium in Mainz, Germany, 31 May to 4 June 2002
- IgG Ratio
- IgM Positive Control Flow HLA Crossmatch
- IL-6 and IL-10 in mice
- IL10-receptor alpha
- IL4 intracellular staining
- Image and Flow Cytometry Position in the UK
- Image Compression
- Image sought
- Immune deficiency Flow position in New Zealand
- Immunodeficiency Flow position in New Zealand
- Immunophenotyping meeting London
- indo-1 excitation at 407 nm?
- INDO-1 storage?
- INF g and TNF b intracellular staining
- INF-g and TNF conjugate for intracellular staining
- inflammatory emails
- injectable dye
- Instability with large sheath tank
- Instability with large sheath tank?
- interesting case
- Interesting laser issue with Coherent Laser
- Interesting T-cell case
- Internalization of antibodies
- Internationalism in ISAC
- intracellular cAMP assay?
- Intracellular Cytokine Staining GFP cells
- Intracellular cytokines
- Intracellular pH
- intracellular staining of membrane protein
- Intracellular Staining with PC5
- Intriguing Flow on Lymphoma Patient
- Inventory databases
- ISAC 2006 location
- ISAC Education Workshop
- ISAC XXI
- ISAC XXI Core Manager's Workshop
- ISAC XXI Technological Advances in Flow and Image Cytometry Workshop
- ISAC-Tutorial antigen-specific cytometry
- Isotype control
- Isotype Gating
- JC-1
- JC-1 summary
- Job Application - DakoCytomation FlowCenter Freiburg Germany
- Job Opening
- Job Opening -- Immediate -- Vaccine Research Center
- job opening at Biogen
- Job Opening in Southern California, USA
- Job Opportunities in Seattle
- job opportunity
- Job Opportunity at StemCells, Inc.
- Job opportunity in Perth Western Australia
- Job Posting
- job posting - Pfizer Global Research and Development
- Job posting, Oxford, UK
- job postings
- jobs and stuff....
- Joe Trotter e-mail address
- Kallestad ??
- Kallestad ?? [FAL-WSXBB13]
- Kallestad follow-up [DC-1VEC3G]
- karyotyping via capillary electrophoresis
- KI67 staining
- Kits for Apoptosis detection
- Kupffer cell Ab labeling
- LAG-3 MAbs
- Langerhan's Cells
- laser change recommendation
- laser chilling
- Laser growing old...
- Laser Power
- laser recommendation
- Laser Scanning Cytometer vs Confocal
- Last Chance for Used HP 340 Systems
- Last Message on Purdue Cytometry CD-ROM Vol 6
- length of storage of fix cells
- Leukocyte Alkaline Phosphatase
- Light scatter/cell size chahges during apoptosis.
- List-mode files
- Liver prep/infiltrating leukocytes
- liver T cells
- LMRP's for flow
- loader
- Locating C-Six Diagnostics
- Log In program
- Login software
- London, UK: Position available
- Long-term GFP expression without selection
- look for commercial or easily shared BCRP and P-gp (mdr1) antibodies?
- Looking for Andrew Beavis
- LOOKING FOR ANTIBODIES
- Looking for antigen internalization protocols for Flow
- looking for CCR7
- Looking for CD52
- looking for Fc fragments
- Looking for flow cytogenetics person at NIH
- Looking for information on five part diffs by Flow
- Looking for Robert McGilp
- Looking for someone
- Looking for websites to post job opportunities
- looking to relocate
- LPS contamination
- LS and Annexin V staining buffer
- luciferase essay using flow cytometer?
- Luminescent metals
- Luminescent Metals responses
- Luminex software
- Luminex?
- lymph node cell separation ?
- Lymph node preps
- Lymphocyte activation
- lymphocyte function assay by flow.
- Lymphocyte subsets reference ranges
- Lymphoma case???
- Lysis of blood cells?
- Mac G3 and OS9.2.2
- Mac security log-in
- macaque Ig isotyping kits
- MacLogin 2.0 question
- MacOS 9 and CellQuest
- Macrophage activation in vitro
- Macrophage harvesting, GIBCO buffer
- Macrophage presence in rat cell line?
- Macrosort Head corrosion
- Macrosort head corrosion - summary
- Magnetic CD8 Beads & Monkeys
- Manager position available
- Many thanks for Fc referrals!
- Margination vs. Decreased Expression
- marker for human coronary artery endothelial cells
- Markers for suppressor macrophages?
- markers to distinguish mouse monocytes
- Mast cells and basic orange 21
- mdr and flow
- meaning of FL2-area and FL-2 width
- Measirung Cell Cycle by Cytometry
- Measuring iNOS activity by FACS
- Medium for FNABs
- Meeting announcement
- meeting reminder
- megakaryocyte staining
- Memory T cells in the rat
- Methanol fixation & APC signal attenuation
- method of blood collection (Thanks and summary)
- MetroFlow meeting update
- mflk-1 antibody
- mflk-1 antibody from Pharmingen
- Microbiology wet workshop at ISAC
- Microglial cell marker
- microscopes wanted
- missing CD69
- Missing Myeloma cells
- mitochondria
- mitochondrial inactivation
- MitoTracker Red (CMXRos) and 3-colour staining
- MK571 and CFDA
- MoAbs to histamine
- MoFlo Facility Manager Needed
- MoFlo operation
- MoFlo vs Vantage
- Monensin vs Brefeldin
- monoclonal B-cells due to amyloidosis
- monoclonal populations in patients with amyloidosis
- monoctes and flow cytometry
- monocyte isolation
- monocyte maturation in bone marrow
- Monocytes and 7-AAD
- Monocytes and macrophages
- Monocytes and Macs
- Monocytes and Macs, AND MORE
- Monocytes Methods anyone?
- monomeric avidin
- more on flies with GFP and Hoechst
- mothballing EPICS V
- mouse adipocytes
- mouse B cells
- mouse CD64 antibody
- mouse hepatocyte isolation
- mouse macrophages
- mouse mast cells
- mouse monocyte progenitors
- Mouse Spleenocytes
- Mouse Suspension Cells
- Moving BrdU peak
- moving to Leiden
- MRK16
- mRNA loss during cell fixation
- MT4 cells
- multi-laser vs single laser charges
- murine memory markers
- Murine mouse cell marker
- Murine platelets
- Murine red blood cells
- My last bit on 256 vs 1024 channels
- my words
- Myeloid populations in myeloproliferative disorders
- Myeloid populations in myeloproliferative disorders/ CD203c
- N/A
- Na-heparin / Li-heparin for isolating lymphocytes
- natural cytotoxicity receptors
- Need a source for anti-mouse CD166
- Need help
- Need Help.."proteasome mediated degradation"
- Need Printer for Quadra 650
- Negative gating and rare events
- negative gating in rare event analysis
- Neuroblastoma removal?
- Neuroblastomas removal?
- neurospora
- neutrophil apoptosis
- neutrophil apoptosis at biomaterial contact
- neutrophils
- new cell cycle journal
- New Cytometry Position at MSKCC in NY
- New Position
- new saphire
- newbie help with software compensation
- NHIC Proposed Local Medical Review Policy - CA
- Nile red compensation
- Nitrogen vs. air in cell sorters
- NK antibodies
- NK cells
- NK cytotoxicity assay with human whole blood
- NK Markers in Cynos
- Non-inhibiting anti human CD62L mab
- non-radioactive LPA
- non-radioactive LPA and responses to query on LPAs
- Non-specific staining of nuclei
- normal values by sex
- Northeast/MidAtlantic Cytometry Symposium April 5-6, 2002
- Nozzle sizes
- NPE cell analyzer hands-on demo at NIH
- Nuclear membrane and phosphatidylserine
- O ring of FACSVanatge sorter
- Oct 4 localisation problem
- OKT6 or CD1a blocking mab
- Old data cartridges
- Old ICRF(UK) Web Pages
- Old Sorter PIctures
- On making this forum more valuable
- On the value of this forum
- Once again CD10 staning: FITC or PE ?
- one M3 or two M3?
- One more survey - summary
- One more survey...
- Open NIH Associate Director position
- Open Operator Position
- opinion to a flow book
- opinion to a flow book PS
- Opportunity in Flow Cytometry
- osteoblast markers
- Oxidative burst and dihydrethidium
- P15 expression in hematopoietic cell lines
- P19 cell death
- P19 sorting
- Paraformaldehyde disposal question
- Part time flow position available - Australia
- PARTEC
- Partec filters]
- Partec Flow cytometers
- Partec instruments
- password software again
- PBMC separation from brain tissue
- PBMC's and cynomolgus monkeys
- PC version of FACs program
- PCNA as proliferation marker
- PCNA staining on monocytes
- PdF positions.
- Pe-Cy 5 and APC
- Pe-Cy5 and APC
- Pe-Cy5 and APC - responses
- peak shift?
- Perforin mAb
- Peripheral blood CD 34 counts used in assessing apheresis collect ions
- Peripheral blood CD 34 counts used in assessing apheresis collections
- Permeabilisation Query.. Thankyou.
- Permeabilising Cells
- Phaser 350 driver for Mac OS X
- PI
- PI washing
- PI washing summary
- PI-8 Ab serach
- Pig Lymphoma
- Pig stem cell marker
- PKC inhibition of intracellular cytokine production in whole blood
- PKH-2 staining of YAC-1
- plant protoplast sorting
- plasma membrane stains
- plate coating with anti-CD40
- Platelet activation
- Platelet Activation Marker: CD62P v
- Platelet Activation Marker: CD62P vs Annexin V
- Platelet activation studies
- Platelet-monocyte aggregates
- Please help: which anti-CD10 clones work well on lymph nodes ?
- pluronic acid
- PM1 cell line
- PMN isolation w/o activation
- PNA probes
- PNH
- Polarization of Fluorescence Signals
- Populations discrimination
- Portugal Course - Braga course in Cytometry
- Position Announcement
- Position available
- Position available at San Diego
- Position Available in Boston
- Position available in Cambridge, UK
- Position available in UK
- Position Description for Flow Cytometry Biologist
- Position in Flow and Image Cytometry at Wyeth Research
- Position Open
- Position Open - Chicago
- Position open/Cell sorter operator
- Position Open: Cytometry R&D Mgr.-Indianapolis
- Position Open: Cytometry Tech in Portland Maine
- Positions
- Positions (2) available
- Positions Available
- Positions Available at Esoterix Inc, New Jersey
- Post-Doctoral Fellowship Opportunity to Study Complement Regulati on in Malaria
- Postdoctoral candidate looking for position
- Postdoctoral Position at Wyeth / Genetics Institute
- postdoctoral position available
- posting to Cytometry email list?
- pregnancy
- pregnancy (heavy sheath fluid boxes)
- pregnancy safety
- Preparation of cell suspension for Flow
- printer for flow?
- Prions in FACscan
- problem markers
- problems with red diod laser-FACS Calibur
- Progenitor colony assay scoring
- Program for September Chesapeake Cytometry Meeting
- Proliferation analysis of T cells in vivo
- Prostaglandin E2
- Prostate volume measurements
- protein conc i cytoplasma/versus nucleus
- protocol for conjugating peptides
- Protocols
- Publishers and Contracts
- Publishers and Contracts - digression
- purification of CD34 cells
- Pyronin Y into APC
- Pyronin Y into APC?
- Quantitative flow in the nucleus
- Quenching of CD4 - APC signal
- query on LPAs: replacing tritiated thymidine with flow
- question
- Question for Cytometry Mailing List!!!
- Quick Width
- R&D Quantikine mRNA Kit experience
- Rabbit antibodies in flow cytometry
- RAM-11 antibody
- Raphael Nunez
- rat blood eosinophils
- Rat granulocytes
- Rat p53 antibody
- rat pancreatic islet cells
- Rat, Pig or Rabbit CD62P, CD41 & CD61 antibodies
- RBC and anti glycoA
- RBC and anti glycophorinA
- RBC label
- RBC phenotyping by flow
- RBC Surface Ag detection
- RBC/retic markers
- re ABC transporter
- Re network
- re Peripheral blood CD 34 counts used in assessing apheresis collections
- re sorting CHO cells
- reagent and cell durability questions
- Recommendations for flow courses
- Reconfiguration of Coulter XL
- red blood cells
- red cell and platelet markers
- Red FLICA
- Red FLICA?
- Red He-Ne laser vs. red diode
- red substrate for beta-galactosidase activity monitoring
- Referencing of anitbodies in articles.
- regarding flowcytometry for CD4 and CD8 analysis
- Registration Deadline for Cytometry Symposium
- relative fluorescence
- Reminder: RESEARCH TRIANGLE CYTOMETRY ASSOCIATION 10-July-2002
- REMINDER: RESEARCH TRIANGLE CYTOMETRY ASSOCIATION 10_APR_2002
- Reply: LOOKING FOR ANTIBODIES
- Reponses to NK cytotoxicity assay (& using frozen PBMCs?)
- Reponses to NK cytotoxicity assay with human whole blood
- Report from ISAC Meeting San Diego
- Request
- request for veterinary internship
- Requests for CyFlow Instrument Demo
- Research flow cytometry job
- Research flow cytometry job again
- RESEARCH TRIANGLE CYTOMETRY ASSOCIATION 10_APR_2002
- RESEARCH TRIANGLE CYTOMETRY ASSOCIATION Thursday, May 23, 2002
- Research Triangle Cytometry Association, 23-October-02, 09:00
- RESEARCH TRIANGLE CYTOMETRY ASSOCIATION, 27-SEP-2002, 9:00AM
- Research Triangle Cytometry Association, June 20, 2002
- resolution of anuploid populations
- Responses to my question about meaning of FL2-area and FL-2 width
- Resposta:CD4 on stimulated human T cells
- Retention of listmode files and paper printouts
- retics by scatter?
- retics on FS X SSC?
- RFP detection
- Roche XE-2100 for Stem Cell Counts
- Réf. : endothelial cells
- Réf. : TNP conjugation
- Sales Position Announcement
- Sales Position Open - Southwest
- Sample differential
- Sample differential, sample concentration, and the effect on abort rate
- Scan on offer
- Scheduling Software
- Scheduling software replies
- search for FlowJo users in Paris
- Search Reopened: Flow Cytometry Mgr.
- searching isac meeting abstracts
- Seeking anti-human Ab...
- Seeking good Anti-Human Ab...
- Seeking use of Vantage with 4-5 color capability
- Seminar in Boston on June 14
- Senior Clinical Flow cytometry position in Auckland, New Zealand
- September Chesapeake Flow Meeting
- serum starve
- Setting up a new flow facility
- setting up a pathogen cell sorter..
- sheath fluid
- sheath fluid: thanks
- Sheduling Software Replies #2
- Short meeting, Leeds University
- side population wtih LSR
- side population wtih LSR/Novel Method
- Silence T cells
- Simple Antibody Staining
- simultaneous screening of 2 cell lines
- single cell preparation
- single cytokine bead assay
- Site for the Biostaus
- Site Update
- Smash and Grab
- SNARF-1 and cell cycle
- So here's another job posting in Australia.
- Software compensation
- Software needed for LKB gamma counter
- soluble CD4
- Some isotypes are stickier than others?
- sort of embrio fish cells
- Sort Sterility
- Sorter available; Position available
- Sorter Comparisons
- Sorter Location Survey
- Sorter Location-Help!
- sorting bacteria
- Sorting CHO cells?
- Sorting Drosophila nuclei with GFP
- sorting dsRed
- sorting fozen murine B cells
- sorting GFP macrophages
- Sorting Granulocytes
- Sorting HEK 293 and establishing clonal cell lines
- sorting islet of Langerhans
- Sorting Issues
- Sorting live cells for DNA content?
- Sorting MDA-MB-231
- Sorting Megakaryocytes
- Sorting Multiple Pathogens
- sorting of CD34 stem cells
- sorting of mitochondria
- Sorting Problems with EPICS Altra
- Sorting problems with EPICS Altra still not solved
- sorting rare events- 1 in a million or more
- source for used FACScans?
- sources for FACS Vantage optical configurations
- SP with LSR
- Spare sheath tank for Vantage
- Spectra Physics Ar+ lasers
- Splitting of sensitive cells
- Stabilisation of bone marrow samples
- staining bacterial membranes
- staining for viability
- staining mast cells
- Staining plasmid protocol
- staining with (1) CD4, CD8, CD3, NK & (2) MHCI, MHCII, B7-1
- Standardizing cytometer using fluorescent microspheres (targe t channeling?)
- Standardizing cytometer using fluorescent microspheres (target channeling?)
- Starch in microalgae
- Static Charge on Sort Collection Tube
- Statistical methods for Expression Analysis
- Stats on Coulter Expo 32
- Stem cell enumeration in rabbits
- stimulation of monocytes: fresh versus frozen
- storage of STAINED then fixed
- Strepavidin beads
- Stromal Derived Factor-1 - intracellular ?
- Summary of BD CBA replies
- Summary: best markers for human monocytes
- summary: cell line for oxidative burst studies
- Summary: Help with Biotinylation
- Summary: reagent and cell durability questions
- Summary: Sorting CHO cells
- supercores
- supercores (OT)
- Surface + Apoptosis detection
- Sweatshops of biotechnology
- Symposium & Workshop 'Faces and phases of dendritic cells'
- Synovial fluid analysis by flow
- SYTO Dyes
- T -Ly, CD44, markers, FCM, ?????
- T cell cytotoxicity kit?
- T cell receptor analysis
- tandem dyes
- Teaching material
- Technical fees CPT 88180
- Technical Fees for CPT 88180
- tetanus toxoid summary
- tetramer staining
- tetramer staining after vaccination
- Tetramers, p17, p24
- text to FCS converter for histograms
- Th1 and Th2 reagents
- Thanks
- Thanks (WAS: Broken connector)
- Thanks for "missing CD69"
- Thanks for FACS 6colors help
- Thanks for help with Accu-Drop/FacsVantage Problem
- thanks, but one more thing...
- thanks: antigen specific T cells
- Thanks; harvesting cultured macrophages
- The Digital "Disadvantage"?
- the future flow cytometry lab
- thymidine block
- timing of immunophenotyping staining
- Tissue Factor
- titering antibodies and other basic questions
- TNP conjugation
- tracking cell division
- tracking cell division, not CFSE
- Transfering stats from WinMDI to MS Excel
- Transplantation/Immunophenotyping Job in New Zealand.
- Tree Shrew Antibodies
- trouble with frozen/thawed monocyte cytokines
- TRYING TO UPDATE DEPARTMENT!
- Tumor cell aggregation problem
- TUNEL assay
- TUNEL assay - summary of causes of variability
- TUNEL assay variability
- TUNEL assay variability, trivial causes
- Turbo-sorting murine bone marrow
- Two Positions
- two questions
- typing of bacteria
- U937 cells
- UK FACS Positions
- UK Position available
- UK Sales Position available
- ultrasound sonification?
- Undergraduate opportunity (Co-op position)
- unusual case
- Upcoming MetroFlow meeting
- Upgrade of FACScan to Macintosh platform
- Upgrade to 8.6
- Urgently need K562 cells
- usage tracking programs - PC's
- Use of automated differential counter for thawed samples
- use of GFP and CFP reporters with a VantageSE
- use of multiple fluorescent cell tracking dyes
- Used FACScan or Caliber
- Using magnetic beads
- UV excitable dyes for surface phenotyping - ELF-97?
- UV laser for sorting SP cells
- UV lasers
- Vacancy-EMBL-Monterotondo.Italy
- Vantage and MacOS software
- Vantage cell counts
- variation of intracellular cytokines within a subject
- Vascular Smooth Muscle Cells
- VEGF-R1 (FLT-1) antibodies for flow?
- Viable Gender markers
- video clips of flow cytometry
- Virus (WORM_KLEZ.E)
- Visible cells in culture
- VLA, CD36, AnnexinV and RBC
- VWF AB
- was "degranulation" now "why flow?"
- was Human IgE, now "why Basotest?"
- was Human IgE, now \"why Basotest?\"
- WASP
- Web site links
- websites for flow cytometry core facilities
- Where is Joe Chrest?
- Which companies make antibodies against for a protein supplied by the consumer ?
- whole blood calcium flux?
- Wich differences between Cell Quest and Flow Jo?
- WinMDI Uninstall
- working on erythrocytes with flow
- Workshop 'Faces and phases of dendritic cells' registration deadline
- X and Y chromosome detection by flow
- xeno reagents
- Y. pestis
- Yeast cell cycle analysis
- Yeast cells, PI and cell cycle
- yeast contaminations
- Yeast DNA analysis with various nozzle diameters
- Yeast DNA analysis with various nozzle diameters (a more spec ific question)
- Yeast DNA staining
- You're joking aren't you Volker??
- Zenon changed my life
- Zenon technology
Last message date: Tue Dec 31 2002 - 11:38:04 EST
Archived on: Sun Jan 05 2003 - 19:26:33 EST
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