Frozen Antibodies

From: O'Donahue, Melanie . (ODonahue.Melanie@MAYO.EDU)
Date: Wed Jul 31 2002 - 10:22:35 EST


Fellow Flow-ers,

Our flow fridge recently went haywire and froze our reagents. We are performing QC on
all of them. So far, they look okay. Does anyone have any experience with the short
and long term effects of freezing antibodies?

On another note, we had a previous operator that kept all of the dry chemicals for
making up reagents such as ammonium chloride lyse, PBS, etc., in the dessicator. The
bottles don't specify this type of storage. Anyone have any thoughts on why the
chemicals should stay in the dessicator?

Thanks,

Melanie O'Donahue, MT, ASCP
Flow Cytometry Lead
Mayo Clinic Scottsdale
Odonahue.Melanie@mayo.edu



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