definitely worth testing, I had someone freeze PE conjugates once (gasp!!!) and to my surprise they were fine. Just be sure to spin out aggregates and then check titer. ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: O'Donahue, Melanie . > Sent: Wednesday, July 31, 2002 11:22 AM > To: Cytometry Mailing List > Subject: Frozen Antibodies > > > Fellow Flow-ers, > > Our flow fridge recently went haywire and froze our reagents. We are > performing QC on > all of them. So far, they look okay. Does anyone have any experience with > the short > and long term effects of freezing antibodies? > > On another note, we had a previous operator that kept all of the dry > chemicals for > making up reagents such as ammonium chloride lyse, PBS, etc., in the > dessicator. The > bottles don't specify this type of storage. Anyone have any thoughts on > why the > chemicals should stay in the dessicator? > > Thanks, > > Melanie O'Donahue, MT, ASCP > Flow Cytometry Lead > Mayo Clinic Scottsdale > Odonahue.Melanie@mayo.edu > >
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