Ondrej, 1. Do you run FACSComp to check your instrument functionality and set the time delay? 2. Regardless of number (1) above, CALL BD and have them come check your instrument. I have one of the "new" red diodes with the board and fan on it and I have had to have it serviced 3 times since installation 8 months ago for drifting out of alignment. Not sure if you have this style of diode, but if so, possibly a bad board on the diode or bad connection. Randy T. Fischer NIH/NIAMS Building 10, Room 6D57 9000 Rockville Pike Bethesda, MD 20892 (301) 594-3537 fischer1@mail.nih.gov > ---------- > From: Ondrej Hrusak > Sent: Monday, April 15, 2002 8:54 AM > To: Cytometry Mailing List > Subject: problems with red diod laser-FACS Calibur > > Dear flowers, > We are having problems with our red laser on FACS Calibur. It turns off > suddenly. Some samples, even within a series of tubes measured with the > same instrument setting, are measured without any signal in the 4th > fluorescence. > > Most annoying of this was our today's finding that it happens even during > one measurement. Thus, we get data that look technically OK (there are > some cells positive in all 4 colors) but according to the percentage, the > number of 4th color-positive cells is low. This is caused by a stretch of > events in channel 1. > > If anyone has experienced this problem, I will appreciate your help. > > Ondrej > > Ondrej Hrusak, M.D., Ph.D. > Institute of Immunology > Ondrej.Hrusak@lfmotol.cuni.cz > fax +4202 2443 5962 > http://www.lf2.cuni.cz/clip/ > phone +4202 2443 5969, +4202 2443 2084 > postal address: V uvalu 84 > 150 06 Praha 5 > Czech Republic >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:07 EST