Dear flowers, We are having problems with our red laser on FACS Calibur. It turns off suddenly. Some samples, even within a series of tubes measured with the same instrument setting, are measured without any signal in the 4th fluorescence. Most annoying of this was our today's finding that it happens even during one measurement. Thus, we get data that look technically OK (there are some cells positive in all 4 colors) but according to the percentage, the number of 4th color-positive cells is low. This is caused by a stretch of events in channel 1. If anyone has experienced this problem, I will appreciate your help. Ondrej Ondrej Hrusak, M.D., Ph.D. Institute of Immunology Ondrej.Hrusak@lfmotol.cuni.cz fax +4202 2443 5962 http://www.lf2.cuni.cz/clip/ phone +4202 2443 5969, +4202 2443 2084 postal address: V uvalu 84 150 06 Praha 5 Czech Republic --
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