I'd like opinions on developing a bead assay for detecting antibody responses by flow cytometry. This assay would be used for assessing immune status of mice immunized with hantaviral nucleocapsid antigen, and eventually we'd like to get it going for humans as well. Right now, the turnaround time for the current test (ELISA) in humans is 2 days, and by that time the patient is either dead or on the road to recovery. I'm planning to coat polystyrene beads from Bangs Labs with recombinant nucleocapsid antigen, then testing serum samples for antibody responses by detecting with anti-IgG-FITC and anti-IgM-PE. My concern lies primarily with coating the beads with the antigens. I was hoping that if anyone on the group has experience with this approach that they might share some of their insights. Thanks, Tony -- Tony Schountz, Ph.D. Dept. of Biology Mesa State College
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