It is true that the TCR is downregulated after activation. But I have found that the effect lasts for only about 24 hours (depending on conditions), so you might see double positives at 48 hours. James F. George, Ph.D. Division of Cardiothoracic Surgery Department of Surgery University of Alabama at Birmingham Birmingham, AL 35294-0007 Phone 205-934-4261 FAX 205-934-5261 jgeorge@uab.edu > > Hi Michal: > TCR are down-modulated on activated T cells, so it's unlikely you'd > see double-positives (tetramer+/IFNg+) after 48 hours of stimulation. > For an example of simultaneous tetramer and intracellular cytokine > staining, see: > Viral Immunology 14(1):59-69, Direct Functional Analysis of > Epitope-Specific CD8+ T Cells in Peripheral Blood, Xiao-Song He, et > al. 2001. > Barry > > Barry Bredt > Core Laboratory Director > UCSF/SFGH General Clinical Research Center > Phone 415-206-5210 > Pager 415-719-0339 > Fax 415-206-8200 > barryb@itsa.ucsf.edu > Mail - UCSF Box 1353, San Francisco, CA 94143-1353 > Shipments - SFGH Bldg 100, Room 249, 1001 Potrero Av, San Francisco, CA 94110 >
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