> ---------- > From: Karatza > Sent: Wednesday, 10 April 2002 20:34 > To: Cytometry Mailing List > Subject: bal and smokers > > Karatza, > > We have found adding 50 uL 0.2% crystal violet (in PBS) to 100 uL BAL > (following other staining) for 1 min, then wash off with PBS will reduce > autofluorescence considerably. > > Greg Hodge PhD > Senior Medical Scientist > Haematology Department > Women's and Children's Hospital > North Adelaide > South Australia 5006 > ph 08 81616089 > fax 08 81616043 > email hodgeg@mail.wch.sa.gov.au > -------------------------------------------------------------------------- > -- > > > Hi all > I am currently working with bal and must admit that sometimes it is a bit > hard to exclude autoflourecence in smokers. > Does anyone have got any ideas? >
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