In addiition to the already mentioned calcein AM assay, functional MDR efflux assays can be performed using other fluorescent PgP substrates such as DIOC2 and Rhodamine123 in conjunction with MDR inhibitors (for example: verapamil). An additional drug based method is to use daunorubicin in a similar way. Expression can be ascertained using commercially available PgP antibodies such as j219 and mrk16 with relevent isotype controls. hope this is of some help. Matthew Gordon Dept Pharmacy and Pharmacology University of Bath m.gordon@bath.ac,uk
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