Greetings to everybody I have recently obtained some intriguing flow data, as described below, and am looking for help to understand its nature. When I perform multi-color staining on spleen or lymph node cells of immunized mice with anti-CD4-FITC plus other antibodies, I can unequivocally identify by flow cytometry a subset (fifteen to 30%) of CD4+ T cells that are positive for B cell markers, including B200, IgM, IgD, MHC Class II, etc. However, if I use same anti-CD4 antibody conjugated to other flurochrome, such as PE or PerCP, than FITC for multi-color staining, the B cell marker-positive subset of CD4 T cells becomes no longer identifiable. Your comments will be greatly appreciated. Best regards Xinjian
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