CFSE

From: joan Kalnitsky (jkalnits@vt.edu)
Date: Tue Oct 15 2002 - 13:13:11 EST

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         I have a client who is using CFSE for the first time.  She is also
staining with cd4/cd8, using PE-Texas red and PE-Cy5 TC.  When we started
setting this up we ran a cd4/8 and things looked fine.  Then we ran a CFSE
only tube and it is fluorescing everywhere.  We can not comp out the CFSE
signal from any of the other PMT's.  Our  PMT setting for CFSE is pretty
low and the peak is set a bit lower than the literature suggests in the
attempt to reduce this "issue".
         Does anyone have any input as to why the CFSE is so bright in all
the other channels??  and if so any suggestions as to how to deal with this
challenge.
         Thanks in advance for all help.
         Joan K.




         Joan Kalnitsky
         Flow Cytometry Lab Supervisor
         VMRCVM
         (540) 231-4115
         FAX 540-231-7367
         jkalnits@vt.edu

         "It is better to serve than to receive."
            B. Borg

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