>Perhaps another (highly intersting) shift-inducing parameter exists >: protein maturation. It is known that newly produced dsRed, a >distant GFP homolog protein has a "greener" emission spectrum than >the mature dsRed-protein. This might be the case for GFP as well ... > >Jan Interesting! Do you have a reference on this? We have used dsRed here only a little so haven't seen this behavior. Thanks. Marty > >Marty Bigos wrote: > >> >>Hi - >> >>Does anyone know of shifts in the emission spectra of the various gfp >>constructs due to intracellular pH, linkage to other proteins, etc.? >>Thanks. >> >> >>Marty >>-- >>Marty Bigos >>Director, Flow Core >>Gladstone Institute of Virology and Immunology >>Building 3 SFGH Rm 509 >>415-695-3832 >> > >-- >Jan Bayer, Ph.D. >Fondation Jean Dausset - CEPH Email : bayer@cephb.fr >27, Rue Juliette Dodu Tel : +33 1 53 72 51 14 >75010 Paris, France Fax : +33 1 53 72 51 28
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