Marty I'm not sure about this. What I do know is that DsRed1 takes about 48hrs for the protein to mature to get full intensity but I'm not aware of spectral shift during this time although it might be true. Try the ClonTech site and look at DsRed2 which discusses the maturation issues since these are at least partly solved with DsRed2. Larry At 07:49 AM 8/13/2002 -0700, you wrote: >>Perhaps another (highly intersting) shift-inducing parameter exists >>: protein maturation. It is known that newly produced dsRed, a >>distant GFP homolog protein has a "greener" emission spectrum than >>the mature dsRed-protein. This might be the case for GFP as well ... >> >>Jan > >Interesting! Do you have a reference on this? We have used dsRed here >only a little so haven't seen this behavior. > >Thanks. > >Marty > >> >>Marty Bigos wrote: >> >>> >>>Hi - >>> >>>Does anyone know of shifts in the emission spectra of the various gfp >>>constructs due to intracellular pH, linkage to other proteins, etc.? >>>Thanks. >>> >>> >>>Marty >>>-- >>>Marty Bigos >>>Director, Flow Core >>>Gladstone Institute of Virology and Immunology >>>Building 3 SFGH Rm 509 >>>415-695-3832 >> >>-- >>Jan Bayer, Ph.D. >>Fondation Jean Dausset - CEPH Email : bayer@cephb.fr >>27, Rue Juliette Dodu Tel : +33 1 53 72 51 14 >>75010 Paris, France Fax : +33 1 53 72 51 28 Larry W. Arnold, Ph.D. Associate Professor Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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