Hello, everyone! I have a colleague who wants to draw blood from a dog, label the erythrocytes with a permanent (or semi-permanent) fluorescent tag, reinfuse the cells into the dog and later (15 minutes) draw blood from the dog again and run the RBCs on the flow cytometer to determine the recovery of previously-labeled cells. The proposal sounded simple enough to me until I started to look for the required stain or monoclonal antibody that would specifically and uniquely stain the RBCs in a sample of whole blood. I haven't found anything yet, so any ideas you might have would be welcomed. Thanks in advance. Rick Meister * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Richard K. Meister Email: meister.1@osu.edu * * The Ohio State University Voice: (614) 292-9716 * * Dept. of Veterinary Biosciences FAX: (614) 292-6473 * * Cytometry Instrumentation Lab * * 1925 Coffey Road * * Columbus, OH 43210 U.S.A. * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
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