Dear Rick, Try using PKH-26 (Sigma) to label the RBCs (you can purify them before putting the label). The transfer of the dye to non labeled cells is minimal. John ----- Original Message ----- From: "Richard K. Meister" <meister.1@osu.edu> To: cyto-inbox Sent: Tuesday, August 13, 2002 7:28 PM Subject: RBC label > > Hello, everyone! > > I have a colleague who wants to draw blood from a dog, label the > erythrocytes with a permanent (or semi-permanent) fluorescent tag, reinfuse > the cells into the dog and later (15 minutes) draw blood from the dog again > and run the RBCs on the flow cytometer to determine the recovery of > previously-labeled cells. The proposal sounded simple enough to me until I > started to look for the required stain or monoclonal antibody that would > specifically and uniquely stain the RBCs in a sample of whole blood. > > I haven't found anything yet, so any ideas you might have would be welcomed. > > Thanks in advance. > > Rick Meister > > * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * > * Richard K. Meister Email: meister.1@osu.edu * > * The Ohio State University Voice: (614) 292-9716 * > * Dept. of Veterinary Biosciences FAX: (614) 292-6473 * > * Cytometry Instrumentation Lab * > * 1925 Coffey Road * > * Columbus, OH 43210 U.S.A. * > * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * >
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