Hello Rick, I've been playing around with labelling RBCs recently, try PKH26 from molecular probes, idealy you need a green laser to excite it optimaly but I seem to remember that it is so bright that the 488 line will do, try 5uM in PBS +Ca/Mg Best regards, Arnold >Hello, everyone! > >I have a colleague who wants to draw blood from a dog, label the >erythrocytes with a permanent (or semi-permanent) fluorescent tag, reinfuse >the cells into the dog and later (15 minutes) draw blood from the dog again >and run the RBCs on the flow cytometer to determine the recovery of >previously-labeled cells. The proposal sounded simple enough to me until I >started to look for the required stain or monoclonal antibody that would >specifically and uniquely stain the RBCs in a sample of whole blood. > >I haven't found anything yet, so any ideas you might have would be welcomed. > >Thanks in advance. > >Rick Meister > _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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