> ---------- > From: Richard K. Meister > Sent: Wednesday, 14 August 2002 1:58 > To: Cytometry Mailing List > Subject: RBC label > > Rick, > > This may be a bit off the mark but I'll say it anyway. Why not stain the > erythrocytes with anti-Glycophorin A PE, (which is reasonably bright on > human rbcs and assuming you can locate a supplier for anti-canine Gly-A), > wash off excess stain. Check to see if cells are bright for Gly-A PE and > compare with unstained sample and the usual FSC vs SSC plot. Reinfuse rbcs > and run new sample on same settings to determine % rbcs with PE signal. > The volume of blood may necessitate large volumes of MoAb but with the > sensitivity of flow you may be able minimise this. > > Regards, > Greg Hodge > --------------------------------------------------------------- > > > Hello, everyone! > > I have a colleague who wants to draw blood from a dog, label the > erythrocytes with a permanent (or semi-permanent) fluorescent tag, > reinfuse > the cells into the dog and later (15 minutes) draw blood from the dog > again > and run the RBCs on the flow cytometer to determine the recovery of > previously-labeled cells. The proposal sounded simple enough to me until > I > started to look for the required stain or monoclonal antibody that would > specifically and uniquely stain the RBCs in a sample of whole blood. > > I haven't found anything yet, so any ideas you might have would be > welcomed. > > Thanks in advance. > > Rick Meister > > * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * > * Richard K. Meister Email: meister.1@osu.edu * > * The Ohio State University Voice: (614) 292-9716 * > * Dept. of Veterinary Biosciences FAX: (614) 292-6473 * > * Cytometry Instrumentation Lab * > * 1925 Coffey Road * > * Columbus, OH 43210 U.S.A. * > * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * >
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