Howdy, I am having a little problem understanding this whole energy transfer issue. Say you had a GFP protein and another protein fused with DS Red. Also say that these two proteins are supposed to interact with each other. The energy should be transferred from high energy to low energy. Or GFP to DS Red. So her comes the questions. Does this strictly result in a loss of GFP fluorescence and a gain in DS Red fluorescence? One of our users went to a lecture that stated that you actually can get a change in emitted wavelength(Unpublished data). In the above example the DS Red would now be Yellow in emission. Is this True? Could this be true for confocal but not for Flow? Do we now need to use a different dichroic to find the double positive cells? Am I totally off on this whole issue? Be nice!!! Thanks in advance for the help, marv
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