Hi Jim, One of the end affects of proteasome inhibition is apoptosis. I've done a bit of work looking at Annexin V staining as well as a number of common apoptosis measurement protocols. Please feel free to email me directly if you have any specific questions. Chris Chris Groves Millennium Pharmaceuticals Inc. 75 Sidney Street Cambridge, MA 02139 617-679-7495 chris.groves@mpi.com > ---------- > From: Phillips, Jim > Reply To: Phillips, Jim > Sent: Monday, May 20, 2002 4:39 PM > To: Cytometry Mailing List > Subject: Need Help.."proteasome mediated degradation" > > > Hello to all, > One of the PI's at the University of Miami wants to know if anyone has done > or, knows a reference on how to measure "PROTEASOME MEDIATED DEGRADATION" by > flow cytometry? Any and all assistance will be greatly appreciated. Thank > you.. > Jim Phillips > University of Miami School of Medicine > >
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