Hello everyone, I've asked a student to perform analysis of CD69 up-regulation (whole blood +CD2/CD2r complex) over the course of first 4 hours with 15 minute snapshots (16 tubes total). I am wondering if we should put the tubes in the fridge or mix them with azide so we could stain all 16 at the end, or, stain/lyse right away? If anyone has done similar assays I would appreciate a comment. It would be a lot easier to just stain everything in one shot. The second issue is - do we start with one tube with 2 mL blood from which we would take out a little bit every 15 min or start with 16 separate smaller volume tubes? Someone commented that taking blood from the single-tube-setup would change the environment for the remaining cells and thus we would get a different result. Maciej -- Maciej S. Simm Blackbelt Champion of Pipetting in the 2-20uL Range Weill Cornell Medical College Pediatrics Hematology/Oncology Immunology Laboratory/Flow Cytometry Unit 525 East 68th Street N-805 New York, NY 10021 voice (212)746 3428 fax (212)746 8573 --
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