Hi Ryan,
I've used the MoFlo to resolve 0.22, 0.47 and 0.75um beads using log
forward and log side scatter signals. No modifications were made to the
instrument. Side scatter was used to trigger the system. I started with the
larger beads first, tweaked things in so I had lots of space between the
bead signal and the trigger threshold noise, and then moved to the smaller
beads and repeated the process.
I couldn't resolve the .22 and .45 beads using forward scatter alone- I
needed to look at the beads on a two parameter plot of forward vs. side
scatter. With this type of plot all three beads were clearly resolved.
Also, this was done with the drop drive on, so sorting is possible.
DakoCytomation does have a forward scatter PMT option available which may
improve the forward scatter resolution of these small particles.
Peter
Peter Lopez
The Aaron Diamond AIDS Research Center
212.448.5188 (office)
212.448.5158 (fax)
212.448.5190 or 5110 (lab)
Ryan Duggan
<rcduggan@midway.uc To: Cytometry Mailing List
hicago.edu> <cytometry@flowcyt.cyto.purdue.edu>
cc:
08/06/2002 01:09 PM Subject: Detection of Nanoparticles
Hello All,
Is it possible to detect particles on the order of 0.1um to 0.3um using
standard flow cytometers? I've done some preliminary trials, and haven't
had much luck (too much background noise, not enough FSC signal). If
anyone has had any experience with this size particles, I'd appreciate
hearing from you. The instruments I have to use are; FACScan, FACSCalibur,
LSR, FACStar Plus, and MoFlo. Also, If there are feasible modifications I
can make to any of these instruments to be able to see this size particles,
that would be helpful. All ideas are welcome.
I've search the archives and found a few similar questions, but no replies.
Thank You,
Ryan
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