You can try for more sensitivity by setting FSC to log. Also, if the particles are fluorescent , you can trigger from this. The second suggestion has worked well in our case. Jim Houston > ---------- > From: Ryan Duggan > Sent: Tuesday, August 6, 2002 12:09 PM > To: Cytometry Mailing List > Subject: Detection of Nanoparticles > > > > Hello All, > > Is it possible to detect particles on the order of 0.1um to 0.3um using > standard flow cytometers? I've done some preliminary trials, and haven't > had much luck (too much background noise, not enough FSC signal). If > anyone has had any experience with this size particles, I'd appreciate > hearing from you. The instruments I have to use are; FACScan, > FACSCalibur, > LSR, FACStar Plus, and MoFlo. Also, If there are feasible modifications I > can make to any of these instruments to be able to see this size > particles, > that would be helpful. All ideas are welcome. > > I've search the archives and found a few similar questions, but no > replies. > > Thank You, > Ryan > >
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