Hey Barb It looks over compensated to me and it appears that the cells positive for the x parameter are autofluorescing hence the diagonal smear. I suppose it could also be bleed though from another channel you haven't shown. How does the unstained sample look? Is there autofluorescence? Which instrument is this done on? Simon Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 >>> <Barbara_Kutzner@hc-sc.gc.ca> - 8/6/02 4:41 PM >>> HI, Recently we've been looking at data from lymphocytes, CD4 and CD8 cells undergoing apoptosis using Annexin V and have noticed that some lymphocytes and CD4 cells exhibit tailing in the bottom right quadrant of the dot plot - it almost looks very badly compensated. The plot attached is data from a sample given a dose of 1.0 Gy. The 0 Gy dose (and Controls) do not exhibit this pattern while samples that have been irradiated with more than 0.2 Gy do show this pattern. Does anyone have any thoughts on what this is from? (See attached file: flow plot.JPG) Thanks. Barb ____________________ Barbara Kutzner Technologist Consumer and Clinical Radiation Protection Bureau Health Canada 775 Brookfield Road, PL 6303B Ottawa, ON K1A 1C1
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