Hello All, Is it possible to detect particles on the order of 0.1um to 0.3um using standard flow cytometers? I've done some preliminary trials, and haven't had much luck (too much background noise, not enough FSC signal). If anyone has had any experience with this size particles, I'd appreciate hearing from you. The instruments I have to use are; FACScan, FACSCalibur, LSR, FACStar Plus, and MoFlo. Also, If there are feasible modifications I can make to any of these instruments to be able to see this size particles, that would be helpful. All ideas are welcome. I've search the archives and found a few similar questions, but no replies. Thank You, Ryan
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