Hello flow people,
I have a question about autofluorescence that Iąd appreciate help with.
If you see a lot of autofluorescence in samples prepared for intracellular
staining (fixation, permeabilisation), is dye exclusion of dead cells a way
to try and solve this?
{ The experiment prep involves enzymatic digestion(with collagenase and
DNAse) and then percoll gradient of lung samples before intracellular
staining.}
Any help or advise and tips is much appreciated,
Joanna
Malaghan Institute
Wellington
NZ
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:17 EST