Dear ELISPOT-flowers, Approximately one month ago I sent this text to the list: In the past period we have seen a rapidly increasing interest in the ELISPOT technique as an alternative for, or addition to intracellular staining. With this e-mail I would like to inventory the interest in ELISPOT from members of this mailing list, especially to find out if there would be an interest for a separate ELISPOT workshop during the ISAC meeting. Our own research application is basically devoted to the measurement of granzyme-B in the ELISPOT technique. I was very pleased with the many responses. To most of the e-mails I have replied personally and with this e-mail I want to inform all of you on the most important matters. The first and most important conclusion is that there is a lot of interest for an ELISPOT workshop. However and unfortunately, a lot of the people who responded have indicated that they will not attend the ISAC meeting (know what you miss! www.isac-net.org <http://www.isac-net.org> ) . We also received some invitations and were even offered locations to organise local workshops, so we decided not to have a workshop during the ISAC . We can also conclude that a major drawback for starting to use the ELISPOT technique is the complexity and labour of the analysis and the counting of the spots. I would like to stress that this issue has been overcome by the use of automated ELISPOT READERS. To convince yourselves I invite you to bring your own ELISPOT plates to the ISAC and have them analysed in our AELVIS 4-plate reader www.aelvis.com <http://www.aelvis.com> . The reader will be available during the ISAC in the Sanquin booth (603). Again I would like to ask your respond if you would like to have an introduction to automated ELISPOT analysis during the ISAC with or without your own plates or if you wish to attend a local ELISPOT workshop (world-wide) later this year. Other suggestions, questions, or remarks are of course also welcome. Best regards, John The first name changes from CLB since its foundation in 1943. The CLB, the Dutch Red Cross Blood Banks, the Federation of Red Cross Blood Banks and the Dr. Karl Landsteiner Foundation have merged into Sanquin (the Sanquin blood supply foundation). Scientific publications from the location CLB will now be published as from Sanquin research at CLB . Products will continue using the brand name CLB since numerous clones have been clustered as CLB-clone. John Voorn Sanquin Reagents Product manager Plesmanlaan 125 j_voorn@clb.nl <mailto:j_voorn@clb.nl> 1066CX AMSTERDAM the Netherlands tel +31-(0)20 5123246 fax +31-(0)20 5123570 web site www.clb.nl <www.clb.nl>
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