Hi, > > > In our lab we routinely isolate monocytes from peripheral blood (by > Percoll). To put in my two cents, I will use the list of Mayumi to > give my > opinion and experience: > > - Percoll: http://memorias.ioc.fiocruz.br/952/3879.html > In our hands the best solution. We perform this routinely and get > puritiesaround 95% +/- 4%. > am i understanding correctly that this is actually a 3 step procedure... 1. spin through lymphoprep 2. spin through percoll 3. one hour adherence or is this wrong? And here are some other possibilities, though i've never used them... 1. centrifugal elutriation 2. a company names 'axis-shield' makes nycodenz and omnipaque that can both be used in a single step isolation procedure. 3. this reference that i'm not certain if its a one or two step process... J Immunol Methods 1991 Nov 22;144(2):247-51 Related Articles, Links A simple method for the purification of human peripheral blood monocytes. A substitute for Sepracell-MN. Denholm EM, Wolber FM. Division of Hematology, Albany Medical College, NY 12208-3479. Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient which matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 10(6) monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN. PMID: 1960422 [PubMed - indexed for MEDLINE] --- Seth L. Ness M.D., Ph.D Fellow in Human Genetics Department of Human Genetics Mount Sinai School of Medicine Phone:212-241-6947 Fax:212-860-3316 sln8@columbia.edu
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