Dear All, We have been having problems with the transport of CFDA in functional assays of MRP1. PBS+gluc is the recommended solution for the assay but our cells do not like it and die rather quickly. The effects of using Hanks in place of this were to significantly reduce the transport of CFDA out of the cells, although the cells were much healthier. Alternatively there is a creatine and Tris buffered PBS solution but we have not tried this. What do others find? Secondly, we have found that MK571 works unreliably as an inhibitor of MRP1 in media + FCS. Does anyone have any experience they would be willing to share about either of these agents? Many thanks, Bryone Bryone J Kuss MBBS, PhD Department of Haematology and Genetic Pathology FMC, Flinders University South Australia 5042 Fax 618 8204 5450
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