Re: FW: sorting rare events- 1 in a million or more

From: Howard Shapiro (hms@shapirolab.com)
Date: Fri Aug 16 2002 - 11:23:14 EST


Jim Leary wrote-

>Having done a little work in this area over the past 20 years or so,
>a few additional references, perhaps not pertinent to Howard but which you
>might find helpful, are:
>
>Leary, J.F.: "Strategies for Rare Cell Detection and Isolation" In: Methods
>in Cell Biology: Flow Cytometry (Edited by Z. Darzynkiewicz, J.P. Robinson,
>H.A. Crissman), vol. 42: pp. 331-358, 1994. [a review with helpful hints of
>rare cell labeling and flow cytometric and cell sorting strategies]
>
>Rosenblatt, J., Hokanson, J.A., McLaughlin, S.R., Leary, J.F.: "A
>Theoretical Basis for Sampling Statistics Appropriate for the Detection and
>Isolation of Rare Cells Using Flow Cytometry and Cell Sorting" Cytometry
>27: 233-238, 1997.
>
>Hokanson, J.A., Rosenblatt, J.I., Leary, J.F. "Some Theoretical and
>Practical Considerations for Multivariate Statistical Cell Classification
>Useful in Autologous Stem Cell Transplantation and Tumor Cell Purging"
>Cytometry 36: 60-70, 1999.

The references I cited as pertinent are pertinent; so are those Jim cited,
and all of the above will be found in the 4th Edition of Practical Flow
Cytometry. I certainly didn't mean to be impertinent.

-Howard



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