Hi Maciej, Using CD14 as a "dump channel" and defining the lower limit of sensitivity relative to MHC disparate control subjects worked well for us. You can find the details in: Hoffmann TK, Donnenberg VS, Friebe-Hoffmann U, Meyer EM Rinaldo CR, DeLeo AB, Whiteside TL, Donnenberg AD. Rapid Communication: Competition of Peptide-MHC Class I Tetrameric Complexes with Anti-CD3 Provides Evidence for Specificity of Peptide Binding to the TCR Complex, Cytometry, 41:321-328, 2000. Albert Donnenberg -----Original Message----- From: Maciej S. Simm [mailto:mss2004@med.cornell.edu] Sent: Wednesday, August 14, 2002 11:54 AM To: cyto-inbox Subject: Tetramers, p17, p24 Hello everyone, I've been asked to establish a protocol for detection of the HIV p17 and p24 specific T cells by tetramer staining. Does anyone have a good "starter" reference for the tetramer technique? as well as a source of purified p17? thanks for any input, Maciej
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