I would appreciate any helpful tips or references regarding the detection and sorting of Drosophila nuclei having GFP expression. I have an Coulter ELITE ESP sorter using an argon laser and 525BP. I sort GFP everyday, but GFP expression seems marginal to none. The investigator claims to see localized GFP in the embryos, but after processing to nuclei the signal is minimal(approx. 3 fold over noise). Has any one had success using anti-GFP antibody with low grade expression? Thanks in advance, Paul Paul L. Hallberg Flow Cytometry Manager KCC CORE Flow Cytometry Facility Thomas Jefferson University 215-503-4556 215-923-0249(fax) Paul.Hallberg@mail.tju.edu
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