We are seeing dramatic differences in light scatter properties of human B lymphoblastoid cell lines following staining in high Ca++ annexin V staining buffer compared to our usual flow buffer. Intensity of both forward and side scatter signals are reduced in the annexin V staining buffer. Any explanations? Don Mosier _______________________________________________________________________________ Donald E. Mosier, PhD, MD Professor Department of Immunology, IMM-7 The Scripps Research Institute 10550 North Torrey Pines Road La Jolla, CA 92037, USA 858 784-9121 phone 858 784-9190 fax This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify Dr. Mosier by telephone or fax.
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