Re: Visible cells in culture

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My guess (on this one as an organic chemist!) is that trypan blue would
stain the cells but not the collagen (which is estremely low in lysines) but
we have never tried it. Moreover, it should characterize live and dead cell
populations within the collagen matrix. Has anyone ever tried that?





Juergen Ostwald wrote:

> Dr. J.Ostwald
> ENT-Clinic
> University Rostock
>
> Dear flowers,
> I want to stain bad looking cells, growing (I hope so, because I can not
> see the cells with phasecontrast or Hoffmanns modulation contrast) in a
> 3-dimensional meshwork of collagen.Would anyone know of a good method to
> stain the cells in culture, thereafter beeing visible in normal light
> microscope or UV-excited (with fluorochromes) and staying alive, if
> possible.
> Thanks for help

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