brefeldin A vs monensin

From: Joanna Roberts (jroberts@malaghan.org.nz)
Date: Sun Jun 30 2002 - 04:49:42 EST


Hello

I have a question about intracellular cytokine staining for flow cytometry.
One of the researchers here wants to look at IL-2 in human PBMCs and is
wonderining if there are any important differences between using monesin or
brefeldin A to trap cytokine intracellularly.
Any advice or tips are much appreciated,
Thanks,

Joanna
--
Joanna Roberts
--
Malaghan Institute of Medical Research
PO Box 7060 Wellington
New Zealand
64 4 389 5096 x 847 (ph)



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