Re: brefeldin A vs monensin

From: maciej simm (simm@cd4cd8.com)
Date: Mon Jul 01 2002 - 13:57:30 EST


Joanna,
see

http://home.wanadoo.nl/flowcytometry/secretion_stop.htm


ms

on 6/30/02 5:49 AM, Joanna Roberts at jroberts@malaghan.org.nz wrote:

Hello

I have a question about intracellular cytokine staining for flow cytometry.
One of the researchers here wants to look at IL-2 in human PBMCs and is
wonderining if there are any important differences between using monesin or
brefeldin A to trap cytokine intracellularly.
Any advice or tips are much appreciated,
Thanks,

Joanna



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