Dave, From my experience on the FACSVantage a high abort rate occurs if the cells are not flowing 'smoothly'. This condition usually exists when cells are 'clumping' rather than staying in single cell suspension. If you are running fixed cells and are filtering cells before sort I don't understand why this would occur. I experienced this kind of abort rate in attempting to sort mouse neurons which were clumping and settling as I sorted. When we treated cells to eliminate this 'clumping' my abort rate dropped significantly. This problem is more apparent when running cells at a higher rate as well. If you see pulsing of spurious events on the pulse display rather than a constant pulse formation than this is the problem. Good Luck David -- David M Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-Room7017 149 Thirteenth street Massachusetts General Hospital-East Charlestown, MA 02129 Tel (617)726-1683 Fax (617)724-3164
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