Re: use of multiple fluorescent cell tracking dyes

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At 10:34 AM +0000 13/11/02, Andrew Herman wrote:
>Dear Flow-ers,
>I have a friend who wishes to identify adoptively transferred
>murine cells approx. 12 hours after injection. He would like to track 2
>separately labelled, or "tagged" cell populations, staining with
>multiple cell surface markers after isolating the cells from the
>recipient, but isn't interested in cell division. Because of the
>experimental setup congenic markers wouldn't be suitable. Am I right in
>thinking that CFSE would be too bright at this timepoint?
>Any info would be greatly appreciated.
>Thanks,
>Andy
>
>----------------------
>Andrew Herman
>Department of Pathology & Microbiology
>University of Bristol



You can always use a lower concentration of CFSE.

Adrian

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