I cannot, at the moment, find the reference --- but there is a problem with trypan blue from certain manufacturers: it doesn't quench external fluorescence even at enormously high concentrations. I believe the conclusion was that it might be a minor contaminant in the trypan blue that is really doing the quenching. Anyway, I certainly used trypan blue at New England clam chowder concentrations and had trouble getting quenching. I switched to using Molecular Probes anti-fluorescein antibodies --- and got good quencing of external fluorescence (but at a price). Then, after the fact, I read the article which explained that I should have looked to a different vendor for my trypan blue. If anyone has the trypan blue reference --- perhaps they could post it to the network. Otherwise, I will keep looking until I find it. Alice Alice L. Givan Director, Englert Cell Analysis Laboratory Norris Cotton Cancer Center Dartmouth Medical School Lebanon, NH 03756 USA Tel 603-650-7661 Fax 603-650-6130 givan@dartmouth.edu
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