Thanks everyone who responded! I am attaching a summary below. Maciej summary: Dear Maciej, I have heard from a colleague that DMSO-differentiated K562 behave like neutrophils. I have no details. Good luck. Maciej, PLB-985 cells can be differentiated into granulocyte-like cells capable of producing a robust oxidative burst. There is also a gp91 knockout, but unfortunately I cannot be sure of the investigator who produced these cells (I think it may be Mary Dinauer). You'll probably find the investigator in PubMed with "PLB" and "CGD" as search parameters. Maciej, the standard cell lines for modelling monocyte/granulocyte oxidative bursts are U937 and HL60. What you need to be aware of is that some clonal lines don't produce superoxide so if you are given some you will need to make sure they were used to study oxidative burst, because some people don't realize this. The cell lines are available from all the cell banks so you can always start your own clonal lines if you have the time and inclination. I've moved on since I worked with these cell lines and my former supervisor has retired so I suspect all his old cell stocks got trashed. You could try contacting some of the groups who study NADPH oxidase, or the role of receptors in activating this enzyme. They might be able to help you. Maciej, We currently use HL60 human promyelocytic cell line for opsonophagocytic assays. We chemically induce differentiation of these cells into either polymorphonuclear cells or monocytic cells. The cell line is available from ATCC.
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