Dear Flow-ers, I have a friend who wishes to identify adoptively transferred murine cells approx. 12 hours after injection. He would like to track 2 separately labelled, or "tagged" cell populations, staining with multiple cell surface markers after isolating the cells from the recipient, but isn't interested in cell division. Because of the experimental setup congenic markers wouldn't be suitable. Am I right in thinking that CFSE would be too bright at this timepoint? Any info would be greatly appreciated. Thanks, Andy ---------------------- Andrew Herman Department of Pathology & Microbiology University of Bristol University Walk Bristol BS8 1TD Tel. +44 117 928 7511 Fax. +44 117 928 7896 A.Herman@bristol.ac.uk
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