The easiest way to do this is to use two different concentrations of CFSE. We routinely do this with our in vivo cytotoxicity assay, and it should serve the same purpose here. Just label one population with 10 fold less CFSE than the first. You'll be able to easily differentiate the two populations upon retrieval. Good luck. kb ------ Keith Bahjat, Ph.D. Scientist, Cancer Vaccines Cerus Corporation Concord, California Office: (925) 288-6120 FAX: (925) 671-9272 e-mail: keith_bahjat@cerus.com ----- Original Message ----- From: "Andrew Herman" <A.Herman@bristol.ac.uk> To: cyto-inbox Sent: Wednesday, November 13, 2002 2:34 AM Subject: use of multiple fluorescent cell tracking dyes > > Dear Flow-ers, > I have a friend who wishes to identify adoptively transferred > murine cells approx. 12 hours after injection. He would like to track 2 > separately labelled, or "tagged" cell populations, staining with > multiple cell surface markers after isolating the cells from the > recipient, but isn't interested in cell division. Because of the > experimental setup congenic markers wouldn't be suitable. Am I right in > thinking that CFSE would be too bright at this timepoint? > Any info would be greatly appreciated. > Thanks, > Andy > > ---------------------- > Andrew Herman > Department of Pathology & Microbiology > University of Bristol > University Walk > Bristol BS8 1TD > Tel. +44 117 928 7511 > Fax. +44 117 928 7896 > A.Herman@bristol.ac.uk > > >
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