Dear All, We have problems in expanding and/or cloning T cells after IFN-gamma beads selection. The recovery rate is very low. Most probably it is due to over-activation of T cells. Does anyone have a protocol on how to dampen T cell activation so that they would regain their abilities to proliferate further? Best regards, Volkan Ozenci M.D., Ph.D. Immune and Gene Therapy Laboratory R8:01, Cancer Center Karolinska (CCK), Karolinska Institutet S-17176, Stockholm, Sweden Fax: +46 8 309195 Office Tel: +46 8 51776876 Lab Tel: +46 8 51776856 Website: www.cck.ki.se/kiessling/index.htm
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