Here are some Ro (distance of 50% efficient transfer) values for chemical dyes. (Alexa Fluor to Alexa Fluor) http://www.probes.com/handbook/tables/1570.html (Alexa Fluor to quenchers) http://www.probes.com/handbook/tables/1569.html fluorescein and the Alexa Fluor 488 dye probably would have similar Ro values. Using one of these dyes as the donor and 488 nm excitation would likely be preferred or 633 with the Alexa Fluor 633 or Alexa Fluor 647 dye as the donor.. Alan Saluk wrote: > Flowers-- > > What fluorochrome pairs have been successfully used for observing > surface colocalization and subsequent FRET. I have had some nice > results using intrinsic CFP to YFP (with 407nm excitation) in > transgenic systems, but I now have a researcher who wants to evaluate > surface protein colocalization in isolated subcellular components. > While there are some obvious ones that come to mind (PE to Cy5--probably > not the most efficient), I was curious if there is any practical > experience to tap into regarding other pairings. My excitation options > are 351nm, 407nm, and the remaining kryton/argon multiline options > (457-647nm). > > See you next month in America's finest city > > Al
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