I have no real experience on autopsy specimens but I'd guess that the specimens should be collected and fixed within 6 hours. We had spleens that were removed during surgery and sat around for 12 hours in saline before coming to the lab for flow. The majority of the cells had auto-lysed and could not be analysed. On 2 other occasions, we had bone marrow aspirates performed in sick patients in ICU, as it turned out just before demise. The marrow was necrotic on morphology and could not be assessed. Both patients had difficulty maintaining their blood pressure. Te Chih Liu National University Hospital Singapore > -----Original Message----- > From: Stetler-Stevenso, Maryalice (NCI) > [mailto:stetler@mail.nih.gov] > Sent: Thursday, 27 June 2002 9:31 > To: Cytometry Mailing List > Subject: autopsy material > > Does any one have experience with autopsy material. I have a lab > interested in doing FACS analysis on lymphocytes isolated from spleen and > marrow post mortem specimens. They would like to sort individual B cells > based upon surface expression of CD19 and other common lymphocyte > antigens. I imagine one could if the autopsy were performed quick enough > but what is quick enough? > > Maryalice Stetler-Stevenson, M.D., Ph.D. > Chief, Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH > > Sometimes you're the windshield, sometimes you're the bug.
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