We have tried doing flow on autopsy material a number of different times, including on material that was freshly collected and put in in tissue culture media without success. There was always a marked degree of autolysis that resulted in horrible background binding of antibodies. -- Brent Wood MD PhD Director, Hematopathology Laboratory University of Washington Medical Center EM: woodbl@u.washington.edu Phone: (206) 598-6199 Fax: (206) 598-6189 > From: "Stetler-Stevenso, Maryalice (NCI)" <stetler@mail.nih.gov> > Date: Thu, 27 Jun 2002 09:31:28 -0400 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: autopsy material > > Does any one have experience with autopsy material. I have a lab interested > in doing FACS analysis on lymphocytes isolated from spleen and marrow post > mortem specimens. They would like to sort individual B cells based upon > surface expression of CD19 and other common lymphocyte antigens. I imagine > one could if the autopsy were performed quick enough but what is quick > enough? > > Maryalice Stetler-Stevenson, M.D., Ph.D. > Chief, Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH > > Sometimes you're the windshield, sometimes you're the bug. >
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