autopsy material

From: Stetler-Stevenso, Maryalice (NCI) (stetler@mail.nih.gov)
Date: Thu Jun 27 2002 - 08:31:28 EST


Does any one have experience with autopsy material. I have a lab interested
in doing FACS analysis on lymphocytes isolated from spleen and marrow post
mortem specimens.  They would like to sort individual B cells based upon
surface expression of CD19 and other common lymphocyte antigens.  I imagine
one could if the autopsy were performed quick enough but what is quick
enough?

Maryalice Stetler-Stevenson, M.D., Ph.D.
Chief, Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH

Sometimes you're the windshield, sometimes you're the bug.




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