Gary - I second Akos's s comment re Cytek. We have two of their systems here and they work quite well. The sheath pressure should be approximately 5 psi. If you don't find any air leaks or problems in the flow system (e.g. you can see a nice drip into the waste when running), then you might give BD service a call. The pressure regulator board has been know to fail on these machines. Marty Marty Bigos Director, Flow Core Gladstone Institute of Virology and Immunology Building 3 SFGH Rm 509 415-695-3832 >Gary, sometimes the plastic tanks develop very small leaks that hard >to detect. Try a new one and see if it makes a difference. We have a >BD FACSFlow system that can use 20 liters tanks. Great addition to >the Calibur BUT you can not buy it. They do not sell it in the US. >Try Cytek (http://www.cytekdev.com). They have a large tank supply >system for the FACSCan/Calibur. > >Best regards, Akos >Biogen > > >Gary.A.Ward@dartmouth.edu (Gary A. Ward) > >05-Jul-2002 10:35 AM > > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > cc: > Subject: Instability with large sheath tank > > > > >Hello Flow Network, > > >I'm looking for advice from anyone with experience using a FACScan or >FACSCalibur with a large sheath tank. We have been experiencing instability >with our fluorescent signals vs time as well as an apparent decrease in >fluorescence intensity. I've checked for air leaks at the tank and believe it >to be stable. I've also checked for air bubbles in the Pall filter and tubing >and these seem to be clear. We routinely clean our system so I don't >believe it >to be dirty. The history of the laser indicates it to be stable. Any other >suggestions? > > >Also what PSI air pressure are users running with the large tanks? > > >Thank you! > > >Gary A. Ward > >H C Englert Cell Analysis Laboratory > >Norris Cotton Cancer Center > >Lebanon, NH USA --
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