Gary, sometimes the plastic tanks develop very small leaks that hard to
detect. Try a new one and see if it makes a difference. We have a BD
FACSFlow system that can use 20 liters tanks. Great addition to the
Calibur BUT you can not buy it. They do not sell it in the US. Try Cytek
(http://www.cytekdev.com). They have a large tank supply system for the
FACSCan/Calibur.
Best regards, Akos
Biogen
Gary.A.Ward@dartmouth.edu (Gary A. Ward)
05-Jul-2002 10:35 AM
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
cc:
Subject: Instability with large sheath tank
Hello Flow Network,
I'm looking for advice from anyone with experience using a FACScan or
FACSCalibur with a large sheath tank. We have been experiencing
instability
with our fluorescent signals vs time as well as an apparent decrease in
fluorescence intensity. I've checked for air leaks at the tank and
believe it
to be stable. I've also checked for air bubbles in the Pall filter and
tubing
and these seem to be clear. We routinely clean our system so I don't
believe it
to be dirty. The history of the laser indicates it to be stable. Any
other
suggestions?
Also what PSI air pressure are users running with the large tanks?
Thank you!
Gary A. Ward
H C Englert Cell Analysis Laboratory
Norris Cotton Cancer Center
Lebanon, NH USA
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