PKH-26 is also fairly toxic to cells, at least in our hands. That's why we use CFSE instead. -Doug Douglas S. Reed, Ph.D. Microbiologist Respiratory & Mucosal Immunity Department of Aerobiology & Product Evaluation Division of Toxinology & Aerobiology U.S. Army Medical Research Institute of Infectious Diseases 1425 Porter Street, Fort Detrick Frederick, MD 21702-5011 301-619-6728 301-619-6911 fax doug.reed@det.amedd.army.mil -----Original Message----- From: Joanne Lannigan [mailto:joannelannigan@virginia.edu] Sent: Monday, July 08, 2002 10:15 AM To: cyto-inbox Subject: RE: tracking cell division, not CFSE Hi Simon: You could try PKH-26 a lipophilic dye which excites at 488 and emits in the red/orange range. It is often used for cell tracking /proliferation assays. I believe it is sold by Sigma. Regards, Joanne Joanne Lannigan, MS Director, Flow Cytometry Core Facility Jordan Hall, Room 7067 P.O. Box 800734 Charlottesville, VA 22908-0734 Office: 434-924-0274 Lab: 434-263-2695 Fax: 434-982-1071 email: joannelannigan@virginia.edu > -----Original Message----- > From: Simon Monard [mailto:smonard@trudeauinstitute.org] > Sent: Tuesday, July 02, 2002 2:31 PM > To: Cytometry Mailing List > Subject: tracking cell division, not CFSE > > > Hi folks > > Has anyone had any success using both CFSE and another dye to track cell > division of > two ex-vivo labelled cell populations.We use CFSE all the time and it > works great, > what we need is another orange or red fluorescing dye that like CFSE will > be retained > by cells in vivo and will allow us to follow the number of cell > divisions. We have > tried Snarf but it seems to be toxic to the cells we are using. The other > dye must > be excitable with either 488nm or 633nm. Any suggestions? > > best wishes > > Simon > > Simon Monard > FACS Lab Manager > Trudeau Institute > Saranac Lake > NY12983 > > Ph 518 891 3080 X352
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