Re: rat blood eosinophils

From: Howard Shapiro (hms@shapirolab.com)
Date: Wed Nov 06 2002 - 16:46:23 EST

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Karim Vermaelen wrote-

>Can anyone recommend an accurate method to count eosinophils in rat
>blood using FACS? Someone in our lab is currently doing manual counts
>using eosin-treated whole blood... quite excruciating indeed

This question comes up on the list every couple of months; I recommend:

1)polarized vs. depolarized scatter and/or

2)autofluorescence and/or

3)weak CD4 positivity

-Howard

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